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Figure 2. The pH-dependence of gene expression of chondrocytes in alginate beads. Relative mRNA levels for AGC1, COL1, COL2, SOX9, HIF1A, and <t>VEGF</t> after 5 days of culture were determined by quantitative RT-PCR and normalized to 18S rRNA. Asterisk indicates p < 0.05 (n ¼ 6).
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Figure 2. The pH-dependence of gene expression of chondrocytes in alginate beads. Relative mRNA levels for AGC1, COL1, COL2, SOX9, HIF1A, and <t>VEGF</t> after 5 days of culture were determined by quantitative RT-PCR and normalized to 18S rRNA. Asterisk indicates p < 0.05 (n ¼ 6).
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Figure 2. The pH-dependence of gene expression of chondrocytes in alginate beads. Relative mRNA levels for AGC1, COL1, COL2, SOX9, HIF1A, and <t>VEGF</t> after 5 days of culture were determined by quantitative RT-PCR and normalized to 18S rRNA. Asterisk indicates p < 0.05 (n ¼ 6).
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VEGFC is upregulated by CRIP1 in GC cells and involved in CRIP1‐mediated lymphangiogenesis and LM. A,B) ELISA showing the effect of CRIP1 overexpression on VEGFC A) and <t>VEGFD</t> <t>B)</t> <t>secretion.</t> C,D) ELISA showing the effect of CRIP1 knockdown on VEGFC C) and VEGFD D) secretion. E,F) RT‐qPCR showing the effect of CRIP1 overexpression E) and knockdown F) on VEGFC mRNA expression. G) Western blot showing the effect of CRIP1 overexpression and knockdown on VEGFC protein expression. H) Western blot showing the expression of CRIP1 and VEGFC in human GC samples. NT, nontumorous tissues; GC, gastric cancer tissues. I) Representative images (left panel) and quantification (right panel) of the Matrigel tube formation assay with human lymphatic endothelial cells (HLECs). HLECs were cultured with conditioned medium derived from GC cells treated as indicated. Scale bars = 200 µm. J) Representative images of enucleated popliteal lymph nodes across groups. K) Histogram analysis of lymph node volumes across groups. L) Ratios of metastatic to total dissected popliteal lymph nodes from mice inoculated with the indicated cells. χ ‐square test was performed to assess the statistical significance in (L). Error bars represent the mean±SD of three independent experiments. * p < 0.05, ** p < 0.01, *** p < 0.001.
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VEGFC is upregulated by CRIP1 in GC cells and involved in CRIP1‐mediated lymphangiogenesis and LM. A,B) ELISA showing the effect of CRIP1 overexpression on VEGFC A) and <t>VEGFD</t> <t>B)</t> <t>secretion.</t> C,D) ELISA showing the effect of CRIP1 knockdown on VEGFC C) and VEGFD D) secretion. E,F) RT‐qPCR showing the effect of CRIP1 overexpression E) and knockdown F) on VEGFC mRNA expression. G) Western blot showing the effect of CRIP1 overexpression and knockdown on VEGFC protein expression. H) Western blot showing the expression of CRIP1 and VEGFC in human GC samples. NT, nontumorous tissues; GC, gastric cancer tissues. I) Representative images (left panel) and quantification (right panel) of the Matrigel tube formation assay with human lymphatic endothelial cells (HLECs). HLECs were cultured with conditioned medium derived from GC cells treated as indicated. Scale bars = 200 µm. J) Representative images of enucleated popliteal lymph nodes across groups. K) Histogram analysis of lymph node volumes across groups. L) Ratios of metastatic to total dissected popliteal lymph nodes from mice inoculated with the indicated cells. χ ‐square test was performed to assess the statistical significance in (L). Error bars represent the mean±SD of three independent experiments. * p < 0.05, ** p < 0.01, *** p < 0.001.
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VEGFC is upregulated by CRIP1 in GC cells and involved in CRIP1‐mediated lymphangiogenesis and LM. A,B) ELISA showing the effect of CRIP1 overexpression on VEGFC A) and <t>VEGFD</t> <t>B)</t> <t>secretion.</t> C,D) ELISA showing the effect of CRIP1 knockdown on VEGFC C) and VEGFD D) secretion. E,F) RT‐qPCR showing the effect of CRIP1 overexpression E) and knockdown F) on VEGFC mRNA expression. G) Western blot showing the effect of CRIP1 overexpression and knockdown on VEGFC protein expression. H) Western blot showing the expression of CRIP1 and VEGFC in human GC samples. NT, nontumorous tissues; GC, gastric cancer tissues. I) Representative images (left panel) and quantification (right panel) of the Matrigel tube formation assay with human lymphatic endothelial cells (HLECs). HLECs were cultured with conditioned medium derived from GC cells treated as indicated. Scale bars = 200 µm. J) Representative images of enucleated popliteal lymph nodes across groups. K) Histogram analysis of lymph node volumes across groups. L) Ratios of metastatic to total dissected popliteal lymph nodes from mice inoculated with the indicated cells. χ ‐square test was performed to assess the statistical significance in (L). Error bars represent the mean±SD of three independent experiments. * p < 0.05, ** p < 0.01, *** p < 0.001.
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VEGFC is upregulated by CRIP1 in GC cells and involved in CRIP1‐mediated lymphangiogenesis and LM. A,B) ELISA showing the effect of CRIP1 overexpression on VEGFC A) and <t>VEGFD</t> <t>B)</t> <t>secretion.</t> C,D) ELISA showing the effect of CRIP1 knockdown on VEGFC C) and VEGFD D) secretion. E,F) RT‐qPCR showing the effect of CRIP1 overexpression E) and knockdown F) on VEGFC mRNA expression. G) Western blot showing the effect of CRIP1 overexpression and knockdown on VEGFC protein expression. H) Western blot showing the expression of CRIP1 and VEGFC in human GC samples. NT, nontumorous tissues; GC, gastric cancer tissues. I) Representative images (left panel) and quantification (right panel) of the Matrigel tube formation assay with human lymphatic endothelial cells (HLECs). HLECs were cultured with conditioned medium derived from GC cells treated as indicated. Scale bars = 200 µm. J) Representative images of enucleated popliteal lymph nodes across groups. K) Histogram analysis of lymph node volumes across groups. L) Ratios of metastatic to total dissected popliteal lymph nodes from mice inoculated with the indicated cells. χ ‐square test was performed to assess the statistical significance in (L). Error bars represent the mean±SD of three independent experiments. * p < 0.05, ** p < 0.01, *** p < 0.001.
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VEGFC is upregulated by CRIP1 in GC cells and involved in CRIP1‐mediated lymphangiogenesis and LM. A,B) ELISA showing the effect of CRIP1 overexpression on VEGFC A) and <t>VEGFD</t> <t>B)</t> <t>secretion.</t> C,D) ELISA showing the effect of CRIP1 knockdown on VEGFC C) and VEGFD D) secretion. E,F) RT‐qPCR showing the effect of CRIP1 overexpression E) and knockdown F) on VEGFC mRNA expression. G) Western blot showing the effect of CRIP1 overexpression and knockdown on VEGFC protein expression. H) Western blot showing the expression of CRIP1 and VEGFC in human GC samples. NT, nontumorous tissues; GC, gastric cancer tissues. I) Representative images (left panel) and quantification (right panel) of the Matrigel tube formation assay with human lymphatic endothelial cells (HLECs). HLECs were cultured with conditioned medium derived from GC cells treated as indicated. Scale bars = 200 µm. J) Representative images of enucleated popliteal lymph nodes across groups. K) Histogram analysis of lymph node volumes across groups. L) Ratios of metastatic to total dissected popliteal lymph nodes from mice inoculated with the indicated cells. χ ‐square test was performed to assess the statistical significance in (L). Error bars represent the mean±SD of three independent experiments. * p < 0.05, ** p < 0.01, *** p < 0.001.
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VEGFC is upregulated by CRIP1 in GC cells and involved in CRIP1‐mediated lymphangiogenesis and LM. A,B) ELISA showing the effect of CRIP1 overexpression on VEGFC A) and <t>VEGFD</t> <t>B)</t> <t>secretion.</t> C,D) ELISA showing the effect of CRIP1 knockdown on VEGFC C) and VEGFD D) secretion. E,F) RT‐qPCR showing the effect of CRIP1 overexpression E) and knockdown F) on VEGFC mRNA expression. G) Western blot showing the effect of CRIP1 overexpression and knockdown on VEGFC protein expression. H) Western blot showing the expression of CRIP1 and VEGFC in human GC samples. NT, nontumorous tissues; GC, gastric cancer tissues. I) Representative images (left panel) and quantification (right panel) of the Matrigel tube formation assay with human lymphatic endothelial cells (HLECs). HLECs were cultured with conditioned medium derived from GC cells treated as indicated. Scale bars = 200 µm. J) Representative images of enucleated popliteal lymph nodes across groups. K) Histogram analysis of lymph node volumes across groups. L) Ratios of metastatic to total dissected popliteal lymph nodes from mice inoculated with the indicated cells. χ ‐square test was performed to assess the statistical significance in (L). Error bars represent the mean±SD of three independent experiments. * p < 0.05, ** p < 0.01, *** p < 0.001.
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VEGFC is upregulated by CRIP1 in GC cells and involved in CRIP1‐mediated lymphangiogenesis and LM. A,B) ELISA showing the effect of CRIP1 overexpression on VEGFC A) and <t>VEGFD</t> <t>B)</t> <t>secretion.</t> C,D) ELISA showing the effect of CRIP1 knockdown on VEGFC C) and VEGFD D) secretion. E,F) RT‐qPCR showing the effect of CRIP1 overexpression E) and knockdown F) on VEGFC mRNA expression. G) Western blot showing the effect of CRIP1 overexpression and knockdown on VEGFC protein expression. H) Western blot showing the expression of CRIP1 and VEGFC in human GC samples. NT, nontumorous tissues; GC, gastric cancer tissues. I) Representative images (left panel) and quantification (right panel) of the Matrigel tube formation assay with human lymphatic endothelial cells (HLECs). HLECs were cultured with conditioned medium derived from GC cells treated as indicated. Scale bars = 200 µm. J) Representative images of enucleated popliteal lymph nodes across groups. K) Histogram analysis of lymph node volumes across groups. L) Ratios of metastatic to total dissected popliteal lymph nodes from mice inoculated with the indicated cells. χ ‐square test was performed to assess the statistical significance in (L). Error bars represent the mean±SD of three independent experiments. * p < 0.05, ** p < 0.01, *** p < 0.001.
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Image Search Results


Figure 2. The pH-dependence of gene expression of chondrocytes in alginate beads. Relative mRNA levels for AGC1, COL1, COL2, SOX9, HIF1A, and VEGF after 5 days of culture were determined by quantitative RT-PCR and normalized to 18S rRNA. Asterisk indicates p < 0.05 (n ¼ 6).

Journal: Journal of orthopaedic research : official publication of the Orthopaedic Research Society

Article Title: Effects of individual control of pH and hypoxia in chondrocyte culture.

doi: 10.1002/jor.20994

Figure Lengend Snippet: Figure 2. The pH-dependence of gene expression of chondrocytes in alginate beads. Relative mRNA levels for AGC1, COL1, COL2, SOX9, HIF1A, and VEGF after 5 days of culture were determined by quantitative RT-PCR and normalized to 18S rRNA. Asterisk indicates p < 0.05 (n ¼ 6).

Article Snippet: One hundred microliter of medium was used for VEGF ELISA according to the manufacturer’s instructions (DVE00; R&D Systems, Abingdon, UK).

Techniques: Gene Expression, Quantitative RT-PCR

Figure 3. Relative VEGF release into the culture medium after 5 days at different pH levels and oxygen tensions, measured by ELISA. Asterisk indicates p < 0.01 (n ¼ 6). Values are normalized to the average of pH 7.4/pO2 5%.

Journal: Journal of orthopaedic research : official publication of the Orthopaedic Research Society

Article Title: Effects of individual control of pH and hypoxia in chondrocyte culture.

doi: 10.1002/jor.20994

Figure Lengend Snippet: Figure 3. Relative VEGF release into the culture medium after 5 days at different pH levels and oxygen tensions, measured by ELISA. Asterisk indicates p < 0.01 (n ¼ 6). Values are normalized to the average of pH 7.4/pO2 5%.

Article Snippet: One hundred microliter of medium was used for VEGF ELISA according to the manufacturer’s instructions (DVE00; R&D Systems, Abingdon, UK).

Techniques: Enzyme-linked Immunosorbent Assay

VEGFC is upregulated by CRIP1 in GC cells and involved in CRIP1‐mediated lymphangiogenesis and LM. A,B) ELISA showing the effect of CRIP1 overexpression on VEGFC A) and VEGFD B) secretion. C,D) ELISA showing the effect of CRIP1 knockdown on VEGFC C) and VEGFD D) secretion. E,F) RT‐qPCR showing the effect of CRIP1 overexpression E) and knockdown F) on VEGFC mRNA expression. G) Western blot showing the effect of CRIP1 overexpression and knockdown on VEGFC protein expression. H) Western blot showing the expression of CRIP1 and VEGFC in human GC samples. NT, nontumorous tissues; GC, gastric cancer tissues. I) Representative images (left panel) and quantification (right panel) of the Matrigel tube formation assay with human lymphatic endothelial cells (HLECs). HLECs were cultured with conditioned medium derived from GC cells treated as indicated. Scale bars = 200 µm. J) Representative images of enucleated popliteal lymph nodes across groups. K) Histogram analysis of lymph node volumes across groups. L) Ratios of metastatic to total dissected popliteal lymph nodes from mice inoculated with the indicated cells. χ ‐square test was performed to assess the statistical significance in (L). Error bars represent the mean±SD of three independent experiments. * p < 0.05, ** p < 0.01, *** p < 0.001.

Journal: Advanced Science

Article Title: CRIP1 Reshapes the Gastric Cancer Microenvironment to Facilitate Development of Lymphatic Metastasis

doi: 10.1002/advs.202303246

Figure Lengend Snippet: VEGFC is upregulated by CRIP1 in GC cells and involved in CRIP1‐mediated lymphangiogenesis and LM. A,B) ELISA showing the effect of CRIP1 overexpression on VEGFC A) and VEGFD B) secretion. C,D) ELISA showing the effect of CRIP1 knockdown on VEGFC C) and VEGFD D) secretion. E,F) RT‐qPCR showing the effect of CRIP1 overexpression E) and knockdown F) on VEGFC mRNA expression. G) Western blot showing the effect of CRIP1 overexpression and knockdown on VEGFC protein expression. H) Western blot showing the expression of CRIP1 and VEGFC in human GC samples. NT, nontumorous tissues; GC, gastric cancer tissues. I) Representative images (left panel) and quantification (right panel) of the Matrigel tube formation assay with human lymphatic endothelial cells (HLECs). HLECs were cultured with conditioned medium derived from GC cells treated as indicated. Scale bars = 200 µm. J) Representative images of enucleated popliteal lymph nodes across groups. K) Histogram analysis of lymph node volumes across groups. L) Ratios of metastatic to total dissected popliteal lymph nodes from mice inoculated with the indicated cells. χ ‐square test was performed to assess the statistical significance in (L). Error bars represent the mean±SD of three independent experiments. * p < 0.05, ** p < 0.01, *** p < 0.001.

Article Snippet: Cell supernatants were collected to quantify the secretion of VEGFC (DVEC00, R&D systems, MN), VEGFD (DVED00, R&D systems, MN), CCL5 (mlbio, Shanghai, China), and TNF‐ α (mlbio, Shanghai, China) via ELISA assay.

Techniques: Enzyme-linked Immunosorbent Assay, Over Expression, Knockdown, Quantitative RT-PCR, Expressing, Western Blot, Tube Formation Assay, Cell Culture, Derivative Assay